Table 2.

Mass of key organs and artery characteristics

	Age (months)
	Control					DMB
	5	18	21	24	27	21	24	27
Mass of key organs
Heart mass (mg)	134 ± 9	174 ± 17*	167 ± 24*	166 ± 23*	170 ± 28*	150 ± 15	178 ± 22	171 ± 25
Brain mass (mg)	397 ± 47	429 ± 41	450 ± 73	468 ± 43*	445 ± 46*	398 ± 78	418 ± 41	434 ± 50
Lung mass (mg)	165 ± 19	192 ± 18	196 ± 32	209 ± 41*	227 ± 25*	183 ± 48	195 ± 30	214 ± 41
Liver mass (g)	1.56 ± 0.21	1.99 ± 0.64	1.77 ± 0.35	1.62 ± 0.44	1.59 ± 0.59	1.50 ± 0.20	1.44 ± 0.45	1.73 ± 0.70
Kidney mass (mg)	395 ± 66	452 ± 45	458 ± 70	432 ± 96	441 ± 47	446 ± 45	475 ± 54	461 ± 55
Spleen mass (mg)	72 ± 8	75 ± 13	84 ± 19	85 ± 38	106 ± 45	64 ± 9	92 ± 35	100 ± 52
Visceral fat mass (g)	0.98 ± 0.42	1.74 ± 0.95*	1.17 ± 0.79	0.79 ± 0.57	0.42 ± 0.25*	0.72 ± 0.58	0.30 ± 0.29	0.21 ± 0.15
Artery characteristics (resting)
Carotid artery	447 ± 31	467 ± 18	486 ± 46	464 ± 79	488 ± 37	480 ± 28	491 ± 36	500 ± 53
diameter (μm)
Aorta diameter (μm)	625 ± 20	611 ± 35	662 ± 45	653 ± 85	716 ± 55*	631 ± 42	741 ± 58	706 ± 79

Data are mean ± SD. n = 7–20 per age per group. Statistics are one-way (effect of age in control mice only) ANOVA with Dunnett’s post hoc test and two-way mixed (age × treatment group) ANOVA with Šídák’s post hoc test.

^*P < 0.05 control vs. 5 month reference group.

^†P < 0.05 DMB vs. control within time point. Abbreviations: DMB, 3,3-dimethyl-1-butanol.