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. Author manuscript; available in PMC: 2023 Oct 27.
Published in final edited form as: Cell. 2022 Oct 18;185(22):4099–4116.e13. doi: 10.1016/j.cell.2022.09.034

Figure 2: Mosquitoes lacking Orco or Ir8a retain individual human preferences.

Figure 2:

(A) Schematic of two-choice olfactometer assay indicating the location of mosquitoes that were not activated, activated but not attracted, or attracted in response to a control trial in which Subject 25 nylons were placed in both stimulus boxes.

(B,C) Stacked bar plots indicate the mean total percent of mosquitoes that were in each category in all trials for Orco (30–40 mosquitoes/trial, n=30–34 trials, *p<0.01, Wilcoxon rank-sum tests with Bonferroni correction comparing each category across the two genotypes) (B) or Ir8a (30–40 mosquitoes/trial, n=31–33 trials, *p<0.0001, Wilcoxon rank-sum tests with Bonferroni correction comparing each category across the two genotypes) (C).

(D) Schematic of two-choice olfactometer assay, indicating the location (purple shading) of all mosquitoes attracted to either stimulus in a control trial in which Subject 25 nylons were placed in both stimulus boxes.

(E,F) Pie charts of trials in which 9 or fewer animals entered either trap were excluded for Orco (E) or Ir8a (F).

(G,H) Percent of mosquitoes of the indicated genotype attracted to the indicated stimuli in the two-choice olfactometer assay. Data from trials that met the inclusion criteria are displayed as violin plots with median indicated by horizontal black lines and the bounds of the violin corresponding to the range (30–40 mosquitoes/trial, n=11–18 trials, except n=29–31 for the Subject 25 vs 25 comparison). Data corresponding to adjacent violin plots labeled with different letters are significantly different (p<0.05, Wilcoxon rank-sum tests with Bonferroni correction).

(I, J) Percent of mosquitoes of the indicated genotype attracted to the indicated stimuli in the two-choice olfactometer assay. Data from trials that met the inclusion criteria are displayed as violin plots with median indicated by horizontal black lines and the bounds of the violin corresponding to the range (30–40 mosquitoes/trial, n=11–22 trials, except n=24–29 for the Subject 25 vs 25 comparison). Data corresponding to adjacent violin plots labeled with different letters are significantly different (p<0.05, Wilcoxon rank-sum tests with Bonferroni correction).