Figure 10.

Autophagy protects against inflammation-induced TJ barrier loss in vivo. [A] Rapamycin protected against LPS [0.2 µg/kg, 16 h]-induced reduction in occludin in mouse colonocytes. Degradation of SQSTM/p62 indicates autophagy induction. [B] Quantification of occludin from panel A. n = 6 per group. [C] Rapamycin protected against LPS-induced increase in inulin flux. n = 6 per group. [D] LPS-induced loss of occludin [green] was prevented by rapamycin. Caveolin-1 [red], nuclei [blue]. White scale bar = 10 µm. [E] Rapamycin protected against TNF-α [0.2 µg/kg, 4 h]-induced loss of colonic TJ barrier. n = 6 per group. [F] Rapamycin prevented the TNF-α-induced increase in the association between occludin [green] and caveolin-1 [red] [white arrows, yellow colour] in WT mice. White scale bar = 10 µm. One-way ANOVA with Tukey’s post-hoc test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.