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. 2022 Oct 11;17(3):433–449. doi: 10.1093/ecco-jcc/jjac148

Figure 11.

Figure 11.

Rapamycin-induced protection against inflammation is autophagy-dependent. [A] Tamoxifen treatment of adult mice with Atg7 floxed and Ubc-CreERT2 alleles resulted in the loss of ATG7 protein in colonic mucosa [Atg7−/− mice] and caused a decrease in constitutive occludin levels in the colonic epithelial cells. [B] Densitometry for occludin levels in Atg7−/− mice. n = 5 per group. [C] Baseline colonic inulin flux in Atg7fl/fl and Atg7−/− mice. n = 3 per group. Rapamycin failed to prevent LPS- [D] and TNF-α [E]-induced increase in colonic inulin flux in Atg7−/− mice. n = 4 per group. [F] In the acute DSS colitis model, Atg7−/− mice showed markedly increased colonic inulin flux compared to Atg7fl/fl DSS and WT DSS mice. n = 3 per group. Student’s t-test or one-way ANOVA with Tukey’s post-hoc test. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, non-significant.