Figure 1. ISL downregulated the level of circ_0002860 in melanoma cells. RT-qPCR was used for circ_0002860 level detection in melanoma (A) tissues and (B) cells. (C, D) Circ_0002860 and CFLAR levels were determined after A875 and SK-MEL-1 cells were incubated with actinomycin D for 0 h, 4 h, 8 h, 12 h and 24 h. (E, F) The quantification of circ_0002860 and CFLAR was performed by RT-qPCR after total RNA was treated with RNase R for 1 h. (G, H) The circ_0002860 expression was assayed using RT-qPCR after treatment of ISL (10 µg/mL, 20 µg/mL, 40 µg/mL, 60 µg/mL, 80 µg/mL) in A875 and SK-MEL-1 cells. *p < 0.05.