Figure 2. ISL impeded proliferation, cell cycle progression, migration and invasion of melanoma cells via inhibiting circ_0002860. (A) The expression analysis of circ_0002860 was performed via RT-qPCR in vector or oe-circ_0002860 transfected A875 and SK-MEL-1 cells. A875 and SK-MEL-1 cells were treated with control, ISL (60 µg/mL), ISL+vector, ISL+oe-circ_0002860. (B) Cell viability examination was performed via CCK-8 assay. (C) Cell proliferation detection was conducted by colony formation assay. The determination of (D) cell apoptosis and (E, F) cell cycle was performed by flow cytometry. The evaluation of (G) migration and (H) invasion was conducted via transwell assay. (I, J) The measurement of EMT-related proteins was performed via western blot. *p < 0.05.