Figure 4. ISL inhibited the melanoma progression by targeting the circ_0002860/miR-431-5p axis. A875 and SK-MEL-1 cells were treated with control, ISL (60 µg/mL), ISL+vector, ISL+oe-circ_0002860, ISL+oe-circ_0002860+miR-NC mimic, ISL+oe-circ_0002860+miR-431-5p mimic. (A) Cell viability and (B) proliferation were detected by CCK-8 assay and colony formation assay. (C) Cell apoptosis and (E, F) cell cycle were assessed by flow cytometry. (F) The migration and (G) invasion abilities were determined by transwell assay. (H, I) EMT markers were examined by western blot. *p < 0.05.