Figure 5. Circ_0002860 sponged miR-431-5p to increase the RAB9A level in melanoma cells. (A) Starbase exhibited the binding site between RAB9A 3’UTR and miR-431-5p. (B, C) The binding between miR-431-5p and RAB9A was identified by dual-luciferase reporter assay. (D, E) The mRNA and protein levels of RAB9A were measured via RT-qPCR and western blot after transfection with miR-NC mimic or miR-431-5p mimic. RAB9A quantification was performed using RT-qPCR and western blot in (F, G) melanoma tissues and (H, I) cells. (J, K) The effect of 60 µg/mL ISL on RAB9A expression was evaluated via RT-qPCR and western blot. (L) RT-qPCR was applied to detect the RAB9A mRNA level in vector, oe-circ_0002860, oe-circ_0002860+miR-NC mimic and oe-circ_0002860+miR-431-5p mimic groups. (M) RAB9A protein level was detected by western blot after transfection of miR-NC mimic or miR-431-5p mimic. (N) RAB9A protein expression was determined by western blot after transfection as Figure 5L. *p < 0.05.