Key resources table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
AQP1, rabbit recombinant monoclonal	Abcam	Ab168387
ATP1A, rabbit polyclonal	Alomone	ANP-001
CD68, rabbit monoclonal	CST	97778
CLIC6, mouse monoclonal	Santa Cruz	365303
ED1/CD68, mouse monoclonal	Millipore	MAB1435
IBA1, goat polyclonal	Thermo	PA5-18039
KCNJ13, mouse monoclonal	Santa Cruz	398810
Ki67, rabbit monoclonal	CST	9129
pNKCC1, rabbit polyclonal	Millipore	ABS 1004
pSPAK, rabbit polyclonal	Millipore	07-2273
RELT, rabbit polyclonal	Novus	NBP2-56851
NKCC1, rabbit monoclonal	CST	85403S
Anti-WNK1	University of Dundee	S079B
Anti-SPAK	University of Dundee	S365D
Anti-NKCC1	University of Dundee	S022D
Anti-ATP1A1	DSHB	a5
Anti-HRP	Pierce	21130
Anti-beta actin	Abcam	Ab8227
CD16-32	Biolegend	Cat#: 101302
CD45-PE/cy7	Biolegend	Cat#: 202207
CD3-647	Biolegend	Cat#: 201408
Alexa Fluor 488	Invitrogen	Cat#: A32723
Alexa Fluor 555	Invitrogen	Cat#: A32816
Alexa Fluor 647	Invitrogen	Cat#: A32849
Bacterial and virus strains
E. Coli+LPS (BD21)	Lucigen	E. cloni EXPRESS BL21(DE3)
E. Coli−LPS (ClearColi)	Lucigen	CLEARCOLI BL21
Biological samples
N/A
Chemicals, peptides, and recombinant proteins
Bumetanide	Sigma-Aldrich	CAS#: 28395-03-1
aCSF	Harvard Apparatus	Item#: 59-7316
Rapamycin	Cayman	Item#: 13346
Critical commercial assays
N/A
Deposited data
Bulk RNA seq	GEO	GSE192946
Mass spectrometry proteomics data	PRIDE	PXD030678
ScRNA seq	GEO	GSE218143
Experimental models: Cell lines
N/A
Experimental models: Organisms/strains
Wistar rats, albino	Charles River	Strain code 003
Oligonucleotides
N/A
Recombinant DNA
N/A
Software and algorithms
Seurat	Hao et al.151	https://satijalab.org/seurat/
Monocle3	Cao et al.152	https://cole-trapnelllab.github.io/monocle3/
Cellchat	Jin et al.82	https://github.com/sqjin/CellChat
Other
N/A