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. 2023 Mar 30;7(4):e0048. doi: 10.1097/HC9.0000000000000048

FIGURE 5.

FIGURE 5

Overexpression of NgBR reduces SREBP2 maturation. (A) Expression of SREBP2 in the liver was detected by immunohistochemical staining with anti-SREBP2 antibody. NC: primary antibody was replaced by rabbit normal IgG. (B) mRNA expression of SREBP2 in liver and LO2 cells was detected by quantitative real-time PCR (qPCR). n=5. (C and D) Protein expression of SREBP2 precursor [SREBP2(p)] and mature SREBP2 [SREBP2(m)] in liver and LO2 cells were detected by Western blot. (E and F) mRNA expression of SCAP, S1P, S2P, and INSIG2 in liver and LO2 cells were detected by qPCR. **p<0.01, n=5. (G) Expression of SCAP protein in liver and LO2 cells were detected by Western blot. (H) LO2 cells were transfected with 50 nmol/L control siRNA (si-NC) or NgBR siRNA (si-NGBR) for 48 hours, followed by determination of SCAP, SREBP2(m), and NgBR protein expression by Western blot. The statistical results of band density analysis were presented in Figure S7 (http://links.lww.com/HC9/A119). Abbreviations: HFD, high-fat diet; ns: not significant.