FIGURE 6.

Overexpression of NgBR reduces lipid synthesis and actives AMPKα⊡ mRNA expression of the genes for lipogenesis and lipolysis in mouse liver were determined by quantitative real-time PCR (qPCR) (A, *p<0.05, **p<0.01, n=5). Protein expression of FASN, SREBP1 precursor (p) and mature form (m) were determined by Western blot (B). (C and D) Expression of the genes for lipogenesis were detected by qPCR and Western blot as indicated. **p<0.01, n=5. (E and F) Expression of pAMPKα and AMPKα in mouse liver and LO2 cells were detected by Western blot. After transfected with control or NgBR expression vectors, LO2 cells were seeded in plates at ~1×10⁴ cells/well and cultured overnight. Then extracellular acidification rate was monitored over the sequential injection of Rot/AA (0.5 μM) and 2-deoxyglucose (50 mM) (G–J). The OCR was monitored over the sequential injection of oligomycin (1 μM), FCCP (1.5 μM) and Rot/AA (0.5 μM) (K, L). (H–J, L) **p<0.01, n=3. LO2 cells transfected with control or NgBR expression vector were treated with compound C (5 μM) for 24 hours, followed by determination of SREBP1(p), pAMPKα, and AMPKα protein expression by Western blot (M), and NgBR, FASN, and SREBP1 mRNA expression by qPCR (N, **p<0.01, n=5) as indicated. The statistical results of band density analysis were presented in Figure S8 (http://links.lww.com/HC9/A119). Abbreviations: HFD, high-fat diet; ns: not significant.