Figure 10.

Transcription factor EB (TFEB) distribution in normal and autosomal dominant polycystic kidney disease (ADPKD) human renal tissues. Serial formalin-fixed paraffin-embedded sections derived from human ADPKD (A, C, and E) and normal human renal (B, D, and F) tissue were subjected to immunohistochemistry using antibodies raised against TFEB (A and B), glycoprotein nonmetastatic melanoma protein B (GPNMB; C and D), or cathepsin D (CTSD; E and F). Compared with normal renal samples (B), a significant increase in TFEB expression was observed in cyst-lining epithelial cells (A) and there was evidence of nuclear translocation. The TFEB downstream targets GPNMB and CTSD were significantly increased in human renal cysts (C and E) compared with normal renal samples (D and F). Scale bars = 100 µm; scale bars in insets = 20 µm. Images are representative of five ×10 fields within each genotype. NHK, normal human kidney.