Table 2.
Details of antibodies and reagents used in this study
| Reagent | Supplier | Cat. No. | Dilution | Assay |
|---|---|---|---|---|
| Primary antibody | ||||
| phospho-rpS6 (Ser235/6) | CST | 4858 | 1:200 | IHC |
| CTSB | CST | 31718 | 1:200 | IHC |
| TFEB | Bethyl Laboratories | A303-673A | 1:1,000 | IHC, 3-D cyst |
| TFEB | Bethyl Laboratories | A303-673A | 1:200 | IF (FFPE) |
| TFE3 | Invitrogen | PA5-54909 | 1:200 | IHC |
| GPNMB | Invitrogen | PA5-42585 | 1:100 | IHC |
| CTSD | CST | 2284 | 1:200 | IHC |
| TFEB | Invitrogen | PA1-31552 | 1:200 | IF (MDCK) |
| LAMTOR4 | CST | 13140 | 1:200 | IF |
| LC3B | Abcam | ab192890 | 1:200 | IF |
| SQSTM1/p62 | CST | 23214 | 1:200 | IF |
| Secondary antibody | ||||
| Gt α rabbit DyLight 488 | Abcam | ab96899 | 1:200 | IF, 3-D cyst |
| Gt α mouse DyLight 488 | Abcam | ab96879 | 1:200 | IF |
| Gt α mouse DyLight 550 | Abcam | ab96872 | 1:200 | IF |
| Gt α rabbit DyLight 550 | Abcam | ab96884 | 1:200 | IF |
| Fluorescent dyes | ||||
| LysoTracker Red | Invitrogen | L7528 | 1:1,000 | IF |
| Acridine Orange | Immunochemistry Technologies | 6130 | 1:1,000 | IF |
3-D, three-dimensional; CST, Cell Signaling Technologies; FFPE, formalin-fixed parafin-embedded; IF, immunofluorescence; IHC, immunohistochemistry; MDCK, Madin-Darby canine kidney.