Figure 1.

MCC950 and esomeprazole inhibition decreased placental NLRP3 mRNA expression and activation but had no significant effect on renal mRNA NLRP3 expression and activation in placental ischemic rats. NLRP3 inhibition also differentially mediates IL33 levels in circulation and in the placenta. Placental NLRP3 mRNA expression (A), renal NLRP3 mRNA (B), placental IL-1β levels (C), and renal IL-1β levels (D). NLRP3 expression was measured using quantitative real-time-PCR and IL-1β levels were measured using a Bio-Plex assay. IL-33 levels were measured in plasma (E) and placenta using an ELISA (F). G: soluble ST2 levels were measured in plasma using ELISA. Data were presented as means ± SD. *P < 0.05 vs. NP + Vehicle, #P < 0.05 vs. RUPP + Vehicle, n = 5–8. NP, normal pregnant; RUPP, reduced uterine perfusion pressure.