Figure 1.

Spectral flow cytometry of murine lung IMs at homeostasis identifies core binary identity defined by FRβ expression. Lung digests from 10-wk-old male mice (n = 4) were assessed by spectral flow cytometry and concatenated for analysis. A: 93,000 IMs underwent dimensional reduction using Uniform Manifold Approximation and Projection (UMAP) of eight macrophage markers. B: fluorescence intensity heatmaps of individual surface markers overlaid on UMAP plot. C: gating strategy to identify FRβ⁺ and FRβ⁻ IMs, FRβ⁻CCR2⁺ IMs and FRβ⁺LYVE1⁺ IMs. D: four populations from C overlaid on UMAP from A. E: proportions of total IMs for each subpopulation identified in C. FRβ, folate receptor β; IM, interstitial macrophage.