Figure 6.

Involvement of Nrf2 and PGC-1α in the effect of Piog on pyroptosis in the OA chondrocytes. (a and b) Western blotting experiments and densitometric analysis. SR was used as a PGC-1α inhibitor. Nrf2-siRNA was used for knocking down the level of Nrf2. GAPDH was used as an internal reference. (c) ELISA was used to measure IL-1β and IL-18 concentrations. (d) CCK8 assays were performed to measure chondrocyte viability. ∗∗P < 0.01, n = 5. (e) The action mode of PPAR-γ under Piog stimulation to protect from pyroptosis-mediated OA cartilage damage.