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. 2023 Mar 22;8(6):e158429. doi: 10.1172/jci.insight.158429

Figure 2. Mitochondrial fusion is critical for the stemness of CSCs.

Figure 2

(A and B) A549 tumor spheres were treated with or without MYLS22 (40 μM) for 12 hours and detected for ALDH1 and OCT4 mRNA expressions by qPCR. Mean ± SEM from 8 independent experiments. (C and D) A549 tumor spheres with or without MYLS22 (40 μM) were detected for sphere formations. Representative and mean ± SEM from 8 independent experiments. Scale bar: 10 μm. (E) Ration of A549 tumor sphere formation at first (G1) and second (G2) generation after MYLS22 (40 μM) treatment. Mean ± SEM from 8 independent experiments. (F and G) A549 tumor spheres with or without MYLS22 (40 μM) were tested for tumor initiation and growth in NSG mice. Mean ± SEM from 6 mice in each group. (H) Treatment with MYLS22 (40 μM) reduced expressions of stem-related genes in A549 CSC tumors. Mean ± SEM from 10 tumors in each group. *P < 0.05, **P < 0.01, ***P < 0.001 with paired t test.