Skip to main content
. 2023 Mar 22;8(6):e158429. doi: 10.1172/jci.insight.158429

Figure 7. Lipogenesis controls the stem-like properties of CSCs.

Figure 7

(A and B) A549 tumor spheres were treated with an increasing dose of C75 and detected for ALDH1 and OCT4 expressions by qPCR after 12 hours. Mean ± SEM from 8 independent experiments. (C) A549 tumor spheres with or without C75 treatment (50 μM) were analyzed for sphere formations. Representative and mean ± SEM from 5 independent experiments. Scale bar: 10 μm. (D) Ration of A549 tumor sphere formation for the first (G1) and second (G2) generations after C75 (50 μM) treatment. Mean ± SEM from 5 independent experiments. (E and F) A549 tumor spheres with or without C75 treatment (50 μM) were analyzed for tumor initiation and growth in NSG mice. Mean ± SEM from 6 mice in each group. (G) A549 tumor spheres with or without C75 treatment (50 μM) plus OPA1 overexpression were analyzed for tumor growth in NSG mice. Mean ± SEM from 6 mice in each group. (H) A549 tumor spheres with or without FASN knockdown were detected for ALDH1 and OCT4 mRNA by qPCR. Mean ± SEM from 6 independent experiments. (I) Knockdown of FASN in A549 tumor spheres inhibited sphere-formations. Mean ± SEM from 8 independent experiments. (J) A549 tumor spheres were transfected using a second FASN siRNA with or without the reintroduction of FASN and were analyzed for expression of the stem-related genes. Mean ± SEM from 8 independent experiments. (K) A549 tumor spheres were treated with or without CPT1 inhibitor Etomoxir (100 μM) and analyzed for expression of the stem-related genes. Mean ± SEM from 5–7 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 with ANOVA plus Tukey’s method (A, B, and J) and paired t test (C–I and K).