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. 2023 Mar 22;8(6):e165604. doi: 10.1172/jci.insight.165604

Figure 6. AdΔAtgl mice have a diminished response to iPTH.

Figure 6

(A) Representative Western blot of ATGL protein in iWAT of control and AdΔAtgl mice. (B) Serum free fatty acids in control and AdΔAtgl mice 30 minutes after being treated with vehicle or PTH (n = 9–11 mice/genotype). (C) Body weights at the conclusion of 6 weeks of vehicle or PTH treatment (n = 6–8 mice/genotype). (D) Representative microCT images for the distal femurs. (E–G) Quantification of trabecular bone volume per tissue volume (BV/TV, E), trabecular number (Tb. N, F), and trabecular thickness (Tb. Th, G) in the distal femur (n = 7–8 mice/genotype). (H) Representative micrographs of calcein and alizarin red incorporation in the trabecular bone compartment used to calculate dynamic indices of bone formation. Original magnification, 40×. (I and J) Quantification of mineral apposition rate (MAR, I) and bone formation rate per bone surface (BFR/BS, J) in the trabecular bone compartment of distal femur (n = 4–6 mice/genotype). All data are represented as mean ± SEM. Data were analyzed by ANOVA with Tukey’s multiple comparisons post hoc test. * P < 0.05.