Table 1.
Kinetic parameters of the interaction between the studied galectins and FGFRs
| KD1 [M] | KD2 [M] | Kon1 [M−1 s−1] | Kon2 [M−1 s−1] | KOff1 [s−1] | KOff2 [s−1] | |
|---|---|---|---|---|---|---|
| FGFR1-Fc | ||||||
| gal-1 [21] | 1.69E−06 | 6.03E−06 | 1.07E+03 | 1.23E+03 | 1.81E−03 | 9.20E−02 |
| gal-3 [21] | 2.60E−06 | 1.34E−08 | 3.68E+04 | 6.12E+04 | 9.66E−03 | 8.21E−02 |
| gal-7 | 9.65E−06 | 3.31E−06 | 4.95E+03 | 2.20E+03 | 5.47E−02 | 6.72E−04 |
| gal-8 | 9.50E−07 | 1.94E−07 | 2.52E+04 | 8.82E+03 | 2.25E−02 | 7.66E−04 |
| gal-8N-CRD | 2.60E−06 | 1.70E−08 | 1.65E+05 | 4.29E+05 | 1.64E−01 | 1.94E−04 |
| FGFR2-Fc | ||||||
| gal-1 | 6.77E−07 | 1.11E−07 | 2.77E+05 | 9.87E+03 | 1.68E−01 | 7.87E−04 |
| gal-3 | 2.22E−05 | 6.51E−05 | 1.45E+03 | 2.21E+03 | 1.03E−03 | 0.40E−01 |
| gal-7 | 7.83E−06 | 1.40E−06 | 1.66E+04 | 3.48E+03 | 1.20E−01 | 2.12E−03 |
| gal-8 | 3.80E−06 | 1.41E−07 | 1.32E+04 | 3.51E+04 | 3.12E−02 | 4.93E−03 |
| FGFR3-Fc | ||||||
| gal-1 | 1.17E−07 | 1.98E−07 | 3.84E+03 | 8.29E+04 | 2.66E−04 | 2.05E−02 |
| gal-3 | 1.165E−04 | 1.18E−04 | 4.13E+03 | 1.87E+05 | 2.05E−02 | 2.14E−02 |
| gal-7 | 2.55E−06 | 4.22E−07 | 4.24E+04 | 5.65E+05 | 1.31E−01 | 5.23E−04 |
| gal-8 | 1.74E−07 | 6.30E−08 | 1.67E+05 | 1.45E+04 | 2.89E−02 | 8.55E−04 |
| FGFR4-Fc | ||||||
| gal-1 | 1.77E−08 | 1.51E−07 | 1.02E+04 | 6.80E+05 | 1.88E−04 | 9.79E−02 |
| gal-3 | 2.67E−05 | 4.27E−06 | 3.83E+02 | 1.55E+04 | 3.42E−03 | 2.90E−02 |
| gal-7 | 2.73E−05 | 3.21E−08 | 6.08E+03 | 2.90E+05 | 4.19E−03 | 1.05E−02 |
| gal-8 | 2.43E−08 | 2.49E−07 | 3.87E+04 | 6.51E+04 | 9.29E−04 | 1.61E−02 |