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. 2023 Apr 3;13:5425. doi: 10.1038/s41598-023-32045-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Co-incubation with sEV from dairy cows identified as high fertile (HF-EXO) and low fertile (LF-EXO) lead to differential gene expression in bovine endometrial epithelial (bEEL) cells. (A) bEEL cells depicts columnal or cuboidal morphology and showed contact inhibition after confluency (at 48-h, 10X). (B,C) Prostaglandin synthase enzyme gene expression, (D,E) anti-inflammatory mediator gene expression (F,I) pro-inflammatory mediator gene expression in bEEL cells. Values are presented as mean ± SEM. Brown-Frosythe and Welch ANOVA test along with Dunnett’s T3 multiple comparison was selected as post-hoc test to identify statistical significance differences between the groups. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.