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. 2022 Nov 24;30(4):885–896. doi: 10.1038/s41418-022-01084-y

Fig. 4. Caspase-8 facilitates effective cellular release of HSV-1 virions.

Fig. 4

A Anti-gD immunofluorescence analysis of wt and caspase-8−/− U937 cells infected with 50 m.o.i. of HSV-1 for 16 h. Nuclei were stained with Hoechst 33342. B Quantitation of anti-gD fluorescence intensity in relation to Hoechst 33342 of 20 individual cells from 3 independent experiments ± SD using ImageJ version 1.53k. Statistical evaluation is by two-sample Student’s t-test, ***p < 0.001. Note that caspase-8−/− cells exhibit an approximatively twofold lower gD immunostaining. C, D Quantitation of virus titres in the supernatant of wt and caspase-8−/− MEFs (B) or U937 cells (C) infected with HSV-1 for 0, 14, 24 and 48 h, as determined by plaque assay on Vero cells. Viral titres are shown as plaque forming unit (PFU) per ml. Caspase-8−/− cells secrete less HSV-1 particles than wt cells. Data represent the means of 3–5 independent experiments ± SD. Statistical evaluation by one-way ANOVA: **p < 0.01, ***p < 0.001.