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. 2022 Nov 24;30(4):885–896. doi: 10.1038/s41418-022-01084-y

Fig. 9. Manipulating autophagy affects the release of virions from HSV-1 infected wt and caspase-8−/− MEFs.

Fig. 9

A, B Quantitation of virus titres by plaque assay in the supernatant of wt (B) and caspase-8−/− MEFs (A) either mock-infected (0 h) or infected with 10 m.o.i. of HSV-1 for 14, 24 and 48 h and either untreated or treated with 0.5 μM wortmannin (A) or 5 μM rapamycin 1 h prior to infection (B). Viral titres are shown as plaque forming unit (PFU) per ml. HSV-1-infected caspase-8−/− cells secrete more viral particles when autophagy is blocked by wortmannin (A). Conversely, HSV-1-infected wt cells release less viral particles when autophagy is activated by rapamycin (B). Data represent the means of 3–5 independent experiments ± SD. Statistical evaluation by one-way ANOVA: ***p < 0.001.