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. 2023 Mar 29;67(2):215–228. doi: 10.1042/EBC20220168

Figure 4. MS-cleavable cross-linkers aid identification.

Figure 4

(A) The DSSO spacer can be cleaved asymmetrically to produce distinctive fragment doublets in MS2. (B) the ‘MS2 method’ is to match cross-linked peptides from the MS2 spectra and later check the matches by confirming that the doublets exist for that combination of peptide masses. (C) The MS3 approach is to gently cleave the cross-linker and reveal the two peptides based on the doublet masses. These can then be further fragmented to identify the individual peptides.