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. 2023 Apr 3;6:361. doi: 10.1038/s42003-023-04735-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b Efficiency of Ampkα RNAi in hemocytes and intestines of shrimp 36 h post injection of siRNA, analyzed using qPCR (a) and western blotting (b). b′ is the statistical analysis of panel b based on three replicates. c, d Expression levels of Vp28 mRNA (c) and protein (d) in hemocytes and intestine of the siGfp and siAmpkα injection groups challenged by WSSV. e Relative expression levels of Ie1 in hemocytes and intestine of Ampkα-RNAi shrimp. f copies of WSSV in the intestine of siGfp and siAmpkα groups after WSSV challenge. g RNA interference efficiency of Ampkβ in hemocytes and intestine of shrimp 36 h post injection, analyzed using qPCR. h, i Expression levels of VP28 at mRNA (h) and protein (i) levels in hemocytes and intestine of Ampkβ-RNAi shrimp challenged by WSSV. j, k The relative expression levels of IE1 (j) in hemocytes and intestine and the number of copies of WSSV (k) in intestine of the shrimp infected by WSSV, analyzed using qPCR. Significant differences were analyzed using Student’s t test, *P < 0.05; **P < 0.01; ***P < 0.001.