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. 2023 Jan 24;30(4):906–921. doi: 10.1038/s41418-022-01108-7

Fig. 5. Gal-7 binding to myeloid cells promotes polarization toward a monocytic profile.

Fig. 5

A WT BM-derived MDSCs were activated with LPS in the presence (LPS + Gal-7) or absence (LPS) of rGal-7 (20 µg/ml) and with or without lactose (30 mM). Representative dot plots (left panel) and quantification (right panels) of the frequencies of MDSCs subpopulations obtained, determined according to Ly6C and Ly6G markers in CD11b+ gated cells (mean ± SEM of 3 independent experiments), are shown. B Binding of Gal-7 to WT BM-derived MDSCs activated or not with LPS, in the presence or absence of lactose, evaluated by flow cytometry. Left panel, representative histogram. Right panel, quantification (mean ± SEM; 4 independent experiments). C Gal-7 binding to non-activated (control) and activated (LPS) BM-derived MDSCs, evaluated by immunofluorescence staining (Gal-7, blue; Ly6C, green; Ly6G, red). Representative images of 3 independent experiments are shown (bar represents 10 µm). Frequencies of MDSCs subpopulations obtained when BM-derived MDSCs from Mgat5−/− (D) and from C2gnt1−/− (E) mice were activated with LPS in the presence or absence of rGal-7 (20 µg/ml) (mean ± SEM of 4 independent experiments).