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. 2023 Apr 3;6:363. doi: 10.1038/s42003-023-04747-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a PC analysis plot of DEG for resting (circle) and stimulated (triangle) memory and naïve T-cell samples. b GSEA plots for indicated gene sets showing the enrichment score for each stimulated MTC subset as compared to stimulated naïve T cells. c Heatmap of normalized enrichment scores (NES) from GSEA of both stimulated and unstimulated MTC compared to respective naïve T cells. d Schematic describing examples of DEG categorization; before and after stimulation (Stim). e Scatter plot showing log₂FC of DEG in unstimulated vs. stimulated MTC. Dots are colored according to DEG category as indicated. f Heatmap showing log₂ transformation of the percent of cells positive for respective cytokine protein expression. g Bar plot showing number of DEG compared to naïve T cells belonging to Expressed, Induced, or Augmented categories for each memory subset. h Gene expression bar plots showing rpkm for indicated genes. Error bars represent ±1 SD from mean in each group. Asterisks indicate relevant significant differences as detected by DESeq2 algorithm.