Fig. 1. Schematic representation of the analysis workflow to elucidate the regulatory role of circadian rhythms under extreme gravity environmental conditions.
a Published sequencing (microarray, RNA-seq, LC-MS) datasets based on skeletal muscle were gathered from open source repositories (ns = number of samples; f: frequency). b Differential rhythmicity (for circadian datasets) and differential expression analysis were carried out after pre-processing of the raw sequencing datasets. Genes of interest were retrieved from KEGG, Reactome, and literature curation related to skeletal muscle-associated pathways and clock-controlled genes.