Figure 1.

Spatial depiction of single-cell sequencing data and annotation of immune cell types and gene distribution in ESCC patients. (A) U-MAP plot showing the sample source of the single-cell data, with each colour representing one sample, including a total of 4 patients; (B) Representative 7 major immune cell types and distribution annotations in ESCC cells, including T cells, B cells, epithelial cells, fibroblasts, mast cells, endothelial cells and myeloid cells; (C) Single-cell sequencing obtained from 7 cell types in the major m6A gene cluster expression distribution maps, including RAMP2, VWF, S100A2, LUM, CD79A and CD3D. outlines the spatial distribution of expression of their major immune cell types. (D) Scale bar graphs further analyze the distribution of immune cell types in four different ESCC patients; (E) Bubble plots further visualize the major m6A epitopes obtained in (B) 7 major immune cell types and distribution annotated expression relationships; (F) Heat map showing the expression of tumor-associated pathway scores for different immune cell populations obtained by the PROGENy method; (G) Single-cell sequencing visualization of m6A signature for gene set enrichment scoring, divided into m6A signature-high and m6A signature-low groups.