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. 2023 Mar 21;10:1060226. doi: 10.3389/fnut.2023.1060226

FIGURE 5.

FIGURE 5

Akt inhibitor IV hindered the restoration of p-eNOS/eNOS and suppression of pro-inflammatory cytokine expressions by PCA in IL-1β-treated endothelial cells. Summarized protein quantification of (A) p-Akt/Akt and (B) p-eNOS/eNOS relative to GAPDH with and without IL-1β (0.5 ng/mL), PCA (100 nM) and Akt inhibitor IV (1 μM) incubation with representative immunoblots (D); n = 5. Summarized quantification of mRNA levels of MCP1 (C), VCAM1 (D), ICAM1 (E), iNOS (F) relative to 36B4 in mBMECs with and without IL-1β (0.5 ng/mL), PCA (100 nM) and Akt inhibitor IV (1 μM) incubation; n = 5 – 6. P-eNOS, eNOS, 140 kDa; p-Akt, Akt, 60 kDa; GAPDH, 37 kDa. Data are presented in means ± SEM; *p < 0.05, **p < 0.01. PCA, protocatechuic acid.