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. 2023 Mar 21;14:1145114. doi: 10.3389/fmicb.2023.1145114

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Copyright © 2023 Li, Zhang, Li and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The determination of cytokines. The prepared spleen cell suspension was obtained from mice 2 weeks of the last vaccination. The levels of (A) IFN-γ and (B) IL-12 and (C) IL-4 and (D) IL-10 in BALB/c mice immunized with TG290 mRNA-LNP vaccine. Data were obtained from triplicate independent experiments and are presented as means ± SD (n = 5). ****p < 0.0001, analyzed by one-way ANOVA multiple comparisons (compare the mean of each group with the mean of every other group).