REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti human CD3 monoclonal antibody (clone: OKT3), Alexa Fluor™ 700	eBioscience	Cat. # 56-0037-42
Anti human CD8a monoclonal antibody (clone: OKT8) APC-eFluor™ 780	eBioscience	Cat. # 47-0086-42
Fixable Viability Dye eFluor 506	eBioscience	Cat. # 65-0866-14
Anti human CD45RA monoclonal antibody (clone: HI100) Super Bright™ 645	eBioscience	Cat. # 64-0458-42
Anti human CD45RO monoclonal antibody (clone: UCHL1) PE-eFluor™ 610	eBioscience	Cat. # 61-0457-42
Anti human CD27 monoclonal antibody (clone: O323) Super Bright™ 780	eBioscience	Cat. # 78-0279-42
Anti human CD57 monoclonal antibody (clone: QA17A04) Brilliant Violet 711™	BioLegend	Cat. # 393328
Anti human IFNγ monoclonal antibody (clone: 4S.B3) FITC	eBioscience	Cat. # 11-7319-82
Anti human TNFα monoclonal antibody (clone: MAb11) PE-Cyanine7	eBioscience	Cat. # 25-7349-82
Anti human IL-2 monoclonal antibody (clone: MQ1-17H12) APC	eBioscience	Cat. # 17-7029-82
Anti human Granzyme B monoclonal antibody (clone: N4TL33) PE	eBioscience	Cat. # 12-8896-42
Fixable Viability Dye eFluor™ 780	eBioscience	Cat. # 65-0865-14
Anti human KIR2D monoclonal antibody (clone: NKVFS1) FITC	Miltenyi Biotec	Cat. # 130-092-687
Anti human CD8a monoclonal antibody (clone: RPA-T8) PerCP-Cyanine5.5	eBioscience	Cat. # 45-0088-42
Anti human CD45 monoclonal antibody (clone: HI30) PE-Cyanine7	eBioscience	Cat. # 25-0459-42
Anti human HLA-A2 monoclonal antibody (clone: BB7.2) PE-Cyanine7	eBioscience	Cat. # 25-9876-42
Anti human HLA-A3 monoclonal antibody (clone: GAP.A3) APC	eBioscience	Cat. # 17-5754-42
Anti human CD3 monoclonal antibody (clone: OKT3) FITC	eBioscience	Cat. # 11-0037-42
Anti human CD4 monoclonal antibody (clone: OKT4) PerCP-Cyanine5.5	eBioscience	Cat. # 45-0048-42
Anti human CD96 (TACTILE) monoclonal antibody (clone: NK92.39) PE-Cyanine7	BioLegend	Cat. # 338415
Anti human CD197 (CCR7) monoclonal antibody (clone: G043H7) Brilliant Violet 421™	BioLegend	Cat. # 353207
Anti human CD7 monoclonal antibody (clone: REA1244) PerCP-Vio® 700	Miltenyi Biotec	Cat. # 130-124-937
Anti mouse CD45.1 monoclonal antibody (clone: A20) FITC	eBioscience	Cat. # 11-0453-82
Anti human IRF4 monoclonal antibody (clone:REA201) PE-Vio770	Miltenyi Biotec	Cat. # 130-100-909
Anti human CD134 (OX40) monoclonal antibody (clone: ACT35) PE	Miltenyi Biotec	Cat. # 130-116-488
Anti human CD25 monoclonal antibody (clone: REA570) APC-Vio770	Miltenyi Biotec	Cat. # 130-123-469
Anti human CD137 monoclonal antibody (clone: 4B4-1) APC	BioLegend	Cat. # 309810
Anti human CD107a monoclonal antibody (clone: REA792) FITC	Miltenyi Biotec	Cat. # 130-111-620
Bacteria and virus strains
WT MCMV (MW97.01)	In house produced	Wagner et al.,48
mCMV-N4	In house produced	Lemmermann et al., 201028
mCMV-YLQPRTFLL	In house produced	N/A
mCMV- GILGFVFTL	In house produced	N/A
GS1783 bacteria	In house produced	Tischer et al., 201049
Biological samples
PBMCs	Collected in this study	N/A
Chemicals peptides and recombinant proteins
Brefeldin A	eBioscience	Cat. # 00-4506-51
Histopaque 1077	Sigma Aldrich	Cat. # 10771
Phorbol myristate acetate (PMA)	InvivoGen	Cat. # tlrl-pma
Ionomycin	InvivoGen	Cat. # inh-ion
YLQPRTFLL peptide	JPT Peptide Technologies	N/A
GILGFVFTL peptide	JPT Peptide Technologies	N/A
PepTivator Influenza A (H1N1) HA	Miltenyi Biotec	130-099-803
PepTivator SARS-CoV-2 Prot_S	Miltenyi Biotec	130-126-701
Critical commercial assays
Intracellular Fixation & Permeabilization Buffer Set	eBioscience	Cat. # 88-8824-00
Super Bright Complete Staining Buffer	eBioscience	Cat. # SB-4401-75
Deposited data
RNA sequencing data	GEO	GEO: GSE202262
Experimental Models: Organisms
Mouse: NSG-HLA:A∗02 transgenic	The Jackson Laboratory	Strain code: 9617
Oligonucleotides
ie2_FLU_fwd (BAC mutagenesis) CTT TCT CTT GAC CAG AGA CCT GGT GAC CGT CAG GAA GAA GAT TCA GCC TCT GAC TAA GGG GAT TTT AGG ATT TGT GTT CAC GCT CTG AGA GGA CAC GAG GAC GCA TCG TGG CCG GAT CTC	Metabion GmbH	N/A
ie2_FLU_rev (BAC mutagenesis) CCT CTT TAT TTA TTG ATT AAA AAC CAT GAC ATA CCT CGT GTC CTC TCA GAG CGT GAA CAC AAA TCC TAA AAT CCC CTT AGT CAG AGG CTG AAT CTT CTT CCG TGA CCA CGT CGT GGA ATG C	Metabion GmbH	N/A
ie2_COVID-19_fwd (BAC mutagenesis) CTT TCT CTT GAC CAG AGA CCT GGT GAC CGT CAG GAA GAA GAT TCA GTG CAG CTT ATT ATG TTA TCT TCA ACC TAG GAC TTT TCT ATT ATG AGA GGA CAC GAG GAC GCA TCG TGG CCG GAT CTC	Metabion GmbH	N/A
CCT CTT TAT TTA TTG ATT AAA AAC CAT GAC ATA CCT CGT GTC CTC TCA TAA TAG AAA AGT CCT AGG TTG AAG ATA ACA TAA TAA GCT GCA CTG AAT CTT CTT CCG TGA CCA CGT CGT GGA ATG C	Metabion GmbH	N/A
Recombinant DNA
mCMV BAC plasmid pSM3fr	In house produced	Tischer et al., 201049
Software and algorithms
Flow Jo	FLOWJO, LLC (Tree Star)	v10.8.1
GraphPad Prism	GraphPad Software	v8.3.0
Cytoscape	Cytoscape.org	v3.9.1
STRING	EMBL	V11.5