FIGURE 2.

X-ray absorbed dose effect on Candida albicans colony formation and growth parameters. (A,B) X-ray induced changes in the C. albicans colony formation. (A) Control sample (non-irradiated control sample), (B) yeast cell sample after irradiation by X-rays a dose of 800 Gy. Two types of yeast colonies, large (yellow circle) and small (red circle), are presented in the sample. (C) The percentage of two types of yeast colonies depending on the absorbed dose. (D) The dependence of C. albicans biomass on the adsorbed dose of X-ray radiation for the non-irradiated control sample and cells exposed to X-rays (800 Gy). The yeast biomass was assessed using the absorbance (OD) at 600 nm. The kinetic curves of yeast growth were fitted with exponential function: OD₆₀₀ = a + be^R₀t, R²>0.994. Parameter R₀ is related to yeast growth rate and measured in h^{– 1}. (E) The X-ray induced decrease in the yeast growth rate. The dose-dependence of yeast growth rate was fitted with exponential function: R₀ = 0.46273 + 0.252e^{−(x/282.57)}, R² = 0.961, d = 282.57 Gy.