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. 2022 Jul 28;108(4):1039–1052. doi: 10.3324/haematol.2021.279301

Figure 1.

Figure 1.

Generation of CD123.ENG T cells expressing iMC, iM, and iC. (A) Schema of vectors. Data throughout the manuscript are represented by the color code of the circles to the left of each construct. (B) Transduction efficiency was determined by CD20 expression (mean% ± standard deviation%: 57.4%±4.1%, n=12, P=ns). (C) Expression of iMC, iM, and iC was determined by western blot for the HA-tag. (D) CD123.ENG protein production by engager T cells plated in the presence of CD123 protein with or without 0.5 nM chemical inducer of dimerization was determined by enzyme-linked immunosorbent assay (n=3, *P<0.05, **P<0.01, T-test). (E, F) T cells were stained 7 days after transduction for CD4, CD8, CD45RO and CCR7 to determine their immunophenotype (n=5). (E) CD4+ and CD8+ T-cell populations. (F) T-cell subsets: naïve-like, CCR7+CD45RO–; central memory (CM), CCR7+CD45RO+; terminally differentiated (emRA), CCR7–CD45RO–; and effector memory (EM), CCR7–CD45RO+). CID: chemical inducer of dimerization; iMC: inducible MyD88 and CD40; iM: inducible MyD88; iC: inducible CD40; ENG: engager; NT: not transduced.