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Characterization of ubiquitinated PTEN. (A) PIP3 hydrolase assay of recombinant and semisynthetic PTEN with 160 μM soluble PIP3. (B) Phosphatase protection assay of PTEN with 25 nM λ-phosphatase. (C) Vesicle pulldown assay of PTEN with PIP2 containing vesicles.
