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. Author manuscript; available in PMC: 2023 Apr 4.
Published in final edited form as: Biochemistry. 2019 Dec 11;58(51):5135–5150. doi: 10.1021/acs.biochem.9b00674

Figure 1.

Figure 1.

Structural comparison of mammalian CDO and P. aeruginosa 3MDO active site. (A) The 1.6 Å X-ray diffraction structure of cys-bound R. norvegicus CDO (PDB entry 4IEV).33 For the sake of clarity, the highly conserved S-H-Y motif containing a post-translationally modified C93–Y157 pair is shown below as an inset. Selected atomic distances are designated by dashed lines. (B) The 2.14 Å X-ray diffraction structure of the P. aeruginosa 3MDO active site (PDB entry 4TLF).32 Fe-coordinated solvent ligands designated by 1–3 (blue) and the S-H-Y motif are illustrated as an inset for comparison to CDO. (C) Side-on view of Pa3MDO illustrating the connectivity between Fe-bound solvents and the S-H-Y proton relay network.