Figure 2.

Wild-type Av MDO $\log \left(k_{\text{cat}}\right)-\text{pH}$ profile for 3mpa reactions (A, ●) as compared to those of H157N (B) and Y159F (C) variants. The $\log \left(k_{\text{cat}}\right)-\text{pH}$ profile for Mm CDO-catalyzed cys reactions is shown in panel A (■). The data for both variants were collected at 37 ± 2 °C due to the decreased value of $k_{\text{cat}}$, whereas wild-type data were collected at 20 ± 2 °C. Equations 1 and 2 were used to fit wild-type and H157N variant $\left(k_{\text{cat}}\right)-\text{pH}$ results, respectively. A standard linear equation was used to fit the pH dependence of data obtained with the Y159F variant. Table 1 summarizes all kinetic parameters obtained from pH-dependent results.