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. 2023 Mar 30;79(Pt 4):345–352. doi: 10.1107/S2059798323002103

Table 1. Crystallization.

Method Hanging-drop vapor diffusion
Plate type 24-well VDX plates
Temperature (K) 293
Protein concentration (mg ml−1) 5
Buffer composition of protein solution 50 mM Tris, 100 mM NaCl pH 7.8
Composition of reservoir solution 0.2 M sodium chloride, 0.1 M HEPES pH 7.3, 18%(w/v) PEG 3350
Volume and ratio of drop 2 µl, 1:1
Volume of reservoir (µl) 500