Fig. 2.

Knockdown of the SLC1A5 glutamine transporter reduces the Meso-1 cell cancer phenotype. A/B Meso-1 cells treated with 3 μg of control- or SLC1A5-siRNA and cell growth and matrigel invasion were monitored. C Meso-1 cells were electroporated with 3 μg of control- or SLC1A5-siRNA and then transfected with 1 μg of EV-Luc or 8xGTIIC-Luc (encodes TEAD response elements) plasmids and luciferase activity was monitored at 24 h. D Meso-1 cells were transfected with 1 μg of EV-Luc or 8xGTIIC luciferase plasmids and then treated with the indicated level of V-9302 or CB839 and at 24 h luciferase activity was measured. E/F/G Effect of SLC1A5 knockdown and CB-839 or V-9302 treatment on SLC1A5 and YAP1/TEAD signaling proteins in Meso-1 cells. The values are mean ± SEM and the asterisks indicate a significant reduction compared to control, n = 3, p < 0.05. Bars = 50 microns in all panels. Cell growth and invasion were monitored as outlined in Materials and Methods.