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. Author manuscript; available in PMC: 2023 Apr 4.
Published in final edited form as: Mol Carcinog. 2022 Dec 23;62(4):438–449. doi: 10.1002/mc.23497

Fig. 3.

Fig. 3

SLC1A5 knockout reduces Meso-1 cell growth and YAP1/TEAD signaling. A/B Cell growth assays show that two independent Meso-1 SLC1A5 knockout cell lines display reduced cell proliferation. C Wild-type Meso-1 cells and two SLC1A5 knockout Meso-1 cell lines were transferted with 1 μg of EV-Luc or 8xGTIIC luciferase reporter vector and after 24 h extracts were prepared to monitor luciferase activity. D/E Extracts of wild-type and SLC1A5 knockout Meso-1 cells were prepared and assayed for the level of YAP1/TEAD and related proteins. The values are mean ± SEM and asterisks indicate a significant reduction in response compares to control, n = 3, p < 0.05. Bars = 50 microns in all panels. Cell growth was monitored a described in Materials and Methods.