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. Author manuscript; available in PMC: 2023 Apr 4.
Published in final edited form as: Mol Carcinog. 2022 Dec 23;62(4):438–449. doi: 10.1002/mc.23497

Fig. 4.

Fig. 4

Glutamine is essential to maintain the cancer phenotype of NCI-Meso-17 cells. A/B NCI-Meso-17 cells were grown as monolayer or spheroid (MCS cell enriched) cultures. Treatment with glutamine-free medium reduces proliferation and spheriod growth. C/D NCI-Meso-17 cells were grown in glutamine-free or 2 mM glutamine containing medium. Treatment with glutamine-free medium reduces invasion and migration. E/F/G Treatment of NCI-Meso-17 cells with V-9302 reduces cell growth, spheroid formation and cell invasion. H/I/J Treatment of NCI-Meso-17 cells with CB-839 (GLS inhibitor) reduces cell growth, spheroid formation and cell invasion. The values are mean ± SEM and asterisks indicate a significant change reduction as compared to control, n = 3, p < 0.05. Bars = 50 microns in all panels. Cell growth, spheroid formation, invasion and migratoin were monitored as outlined in Materials and Methods.