Fig. 2.

A SDS-PAGE analysis of purified TwCel5. Lane 1, standard molecular weight markers (kDa); lane 2, TwCel5_CBM; lane 3, TwCel5_CAT. B Screening of hydrolytic activity on three cellulosic 0.5% (w/v) substrates. C Comparative progress curves for the degradation of 0.5% (w/v) PASC. Reactions were performed in a 50 mM sodium phosphate buffer (pH 7.5) containing 0.5 M NaCl and 1 µM enzyme incubated at 30 ºC. Hydrolytic activities were determined by measuring the release of reducing sugars using DNSA assay