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. 2023 Apr 4;16:60. doi: 10.1186/s13068-023-02301-7

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Fed-batch fermentation for isoprenyl acetate production. A Isoprenyl acetate accumulation over 144 h by selected production strains, achieving significant titer improvements with each optimization. B Biomass accumulation was significantly lower in the acetate pathway knockout strains JBEI-227452 and JBEI-227476 compared to their DH1 counterparts (JBEI-137161 and JBEI-227474), however C acetate pathway knockouts and acs_se.^L641P provided significant, additive reductions in acetate titer and improvements in isoprenyl acetate titer (n = 3)