Table 1.
List of plasmids and strains used in this study
| Plasmid or strain | JBEI Registry Part ID | Relevant genotype | Refs. |
|---|---|---|---|
| Plasmid | |||
| pMTSA | JBEI-17081 | pA5c-AtoB-HMGS_Sa-HMGR_Sa | [49] |
| IPPBy | JBEI-17844 | ptrc99a-PMD_Sc_HKQ-MKmm | [15] |
| IPPBy-ATF1 | JBEI-136483 | ptrc99a-PMD_Sc_HKQ-MKmm-Atf1_Sc | This work |
| IPPBy-ATF2 | JBEI-136484 | ptrc99a-PMD_Sc_HKQ-MKmm-Atf2_Sc | This work |
| IPPBy-SAAT | JBEI-231873 | ptrc99a-PMD_Sc_HKQ-MKmm-SAAT | This work |
| JBEI-6829 | JBEI-6829 | pA5c-AtoB-HMGS_Sa-HMGR_Sa-MKco-PMKco | [33] |
| NudBR10-PMD | JBEI-15848 | pTrc99A-NudBR10-PMD_Sc | [39] |
| pDNC1 | JBEI-231823 | pTrc99A-IDI_EcR1-NudBR10-PMD_Sc | This work |
| pDNC2 | JBEI-231824 | pTrc99A-IDI_EcR2-NudBR10-PMD_Sc | This work |
| pDNC3 | JBEI-231867 | pTrc99A-NudBR10-IDI_EcR1-PMD_Sc | This work |
| pDNC4 | JBEI-231868 | pTrc99A-NudBR10-IDI_EcR2-PMD_Sc | This work |
| pDNC5 | JBEI-231869 | pTrc99A-IDI1_ScR1-NudBR10-PMD_Sc | This work |
| pDNC6 | JBEI-231870 | pTrc99A-IDI1_ScR2-NudBR10-PMD_Sc | This work |
| pDNC7 | JBEI-231871 | pTrc99A-NudBR10-IDI1_ScR1-PMD_Sc | This work |
| pDNC7-SAAT | JBEI-231879 | pTrc99A-NudBR10-IDI1_ScR1-PMD_Sc-LacUV5-SAAT | This work |
| pDNC7-ATF1 | JBEI-231880 | pTrc99A-NudBR10-IDI1_ScR1-PMD_Sc-LacUV5-ATF1 | This work |
| pDNC8 | JBEI-231872 | pTrc99A-NudBR10-IDI1_ScR2-PMD_Sc | This work |
| pDNC9 | JBEI-231874 | pBbE1k-ldhA_Ec-pct_Ap | This work |
| pDNC10 | JBEI-231875 | pBbE1k-ldh_Lm-pct_Ap | This work |
| pSAAT | JBEI-231763 | pBbE1k-SAAT | This work |
| pACSse | JBEI-231876 | pS5k-ACS_Se_L641P | This work |
| pACSec | JBEI-231877 | pS5k-ACS_Ec | This work |
| Strain | |||
| DH1 | – | F– λ– endA1 recA1 relA1 gyrA96 thi-1 glnV44 hsdR17(rK–mK–) | Wild type |
| AceKO | JBEI-3606 | DH1 Δpta, ΔackA, ΔpoxB | [50] |
| IPPBy | JBEI-137165 | DH1 harboring JBEI-17081, JBEI-17844 | [15] |
| DH1-IPPBy- ATF1 | JBEI-137161 | DH1 harboring JBEI-17081, JBEI-136483 | This work |
| DH1-IPPBy-ATF2 | JBEI-137162 | DH1 harboring JBEI-17081, JBEI-136484 | This work |
| DH1-IPPBy-SAAT | JBEI-227453 | DH1 harboring JBEI-17081, pDNC33 | This work |
| Original-MVA | JBEI-16126 | DH1 harboring JBEI-6829, JPUB-004507; nudBRBS10 | [39] |
| DNC1 | JBEI-231891 | DH1 harboring JBEI-6829, pDNC1 | This work |
| DNC2 | JBEI-231892 | DH1 harboring JBEI-6829, pDNC2 | This work |
| DNC3 | JBEI-231893 | DH1 harboring JBEI-6829, pDNC3 | This work |
| DNC4 | JBEI-231894 | DH1 harboring JBEI-6829, pDNC4 | This work |
| DNC5 | JBEI-231895 | DH1 harboring JBEI-6829, pDNC5 | This work |
| DNC6 | JBEI-231896 | DH1 harboring JBEI-6829, pDNC6 | This work |
| DNC7 | JBEI-231897 | DH1 harboring JBEI-6829, pDNC7 | This work |
| DNC8 | JBEI-231898 | DH1 harboring JBEI-6829, pDNC8 | This work |
| DNC7-ATF1 | JBEI-231907 | DH1 harboring JBEI-6829, pDNC7-ATF1 | This work |
| DH1-IPPBy-ATF1-Acs_Ec | JBEI-227454 | DH1 harboring JBEI-17081, IPPBy-ATF1, pACSec | This work |
| DH1-IPPBy-ATF1-Acs_Se | JBEI-227452 | DH1 harboring JBEI-17081, IPPBy-ATF1, pACSse | This work |
| 3606-IPPBy-ATF1 | JBEI-227474 | JBEI-3606 harboring JBEI-17081, IPPBy-ATF1 | This work |
| 3606-IPPBy-ATF1-Acs_Ec | JBEI-227475 | JBEI-3606 harboring JBEI-17081, IPPBy-ATF1, pACSec | This work |
| 3606-IPPBy-ATF1-Acs_Se | JBEI-227476 | JBEI-3606 harboring JBEI-17081, IPPBy-ATF1, pACSse | This work |
| 3606-IPPBy-ATF2 | JBEI-231881 | JBEI-3606 harboring JBEI-17081, IPPBy-ATF2 | This work |
| 3606-IPPBy-SAAT | JBEI-231882 | JBEI-3606 harboring JBEI-17081, IPPBy-SAAT | This work |
| DH1-IPPBy-ATF2-Acs_Ec | JBEI-231883 | DH1 harboring JBEI-17081, IPPBy-ATF2, pACSec | This work |
| DH1-IPPBy-ATF2-Acs_Se | JBEI-231885 | DH1 harboring JBEI-17081, IPPBy-ATF2, pACSse | This work |
| DH1-IPPBy-SAAT-Acs_Ec | JBEI-231884 | DH1 harboring JBEI-17081, IPPBy-SAAT, pACSec | This work |
| DH1-IPPBy-SAAT-Acs_Se | JBEI-231886 | DH1 harboring JBEI-17081, IPPBy-SAAT, pACSse | This work |
| DH1-IPPBy-SAAT-ldha_Ec | JBEI-231903 | DH1 harboring JBEI-17081, IPPBy-SAAT, pDNC9 | This work |
| DH1-OriMVA-SAAT-ldha_Ec | JBEI-231905 | DH1 harboring JBEI-6829, pDNC7-SAAT, pDNC9 | This work |
| DH1-IPPBy-SAAT-ldh_Lm | JBEI-231904 | DH1 harboring JBEI-17081, IPPBy-SAAT, pDNC10 | This work |
| DH1-OriMVA-SAAT-ldh_Lm | JBEI-231905 | DH1 harboring JBEI-6829, pDNC7-SAAT, pDNC10 | This work |
| 3606-IPPBy-SAAT-ldhA_Ec | JBEI-231899 | JBEI-3606 harboring JBEI-17081, IPPBy-SAAT, pDNC9 | This work |
| 3606-OriMVA-SAAT-ldhA_Ec | JBEI-231901 | JBEI-3606 harboring JBEI-6829, pDNC7-SAAT, pDNC9 | This work |
| 3606-IPPBy-SAAT-ldh_Lm | JBEI-231900 | JBEI-3606 harboring JBEI-17081, IPPBy-SAAT, pDNC10 | This work |
| 3606-OriMVA-SAAT-ldh_Lm | JBEI-231902 | JBEI-3606 harboring JBEI-6829, pDNC7-SAAT, pDNC10 | This work |
| DH1-SAAT-ldhA_Ec | JBEI-231889 | DH1 harboring pSAAT, pDNC9 | This work |
| DH1-SAAT-ldh_Lm | JBEI-231890 | DH1 harboring pSAAT, pDNC10 | This work |