Fig. 5.

AAV-miR-325-3p OE alleviates lumbar facet joint cartilage senescence in standing model mice. A In situ hybridization of miR-325-3p (red) and cell nucleus (blue) staining of sham, AAV-NC-treated groups, and AAV-miR-325-3p OE-treated groups in standing 10 weeks mice. B Quantification of the percentage of positive miR-325-3p cells in cartilage area in A. C Representative histological images of LFJ cartilage with Hematoxylin–eosin (H&E) and Safranin O/Fast Green after 10 weeks standing in sham, AAV-NC-treated groups, and AAV-miR-325-3p OE-treated groups. D Quantitative analysis of Osteoarthritis Research Society International (OARSI) score in C. E, F Representative immunohistochemistry images of P53 and P21 in LFJ cartilage in sham, AAV-NC-treated groups, and AAV-miR-325-3p OE-treated groups. G, H Quantitative analysis of P53 and P21 in E and F. Scale bar = 50 μm. n = 6 per group. All data are shown as the mean ± standard deviation (SD). *P < 0.05, **P < 0.01