Table 1. Macromolecule-production information.

Structure	L702H/H875Y/F877L/T878A	L702H/H875Y/F877L	H875Y/F877L/T878A	F877L/T878A	L702H/H875Y
Source organism	Homo sapiens
DNA source	cDNA
Forward cloning primer	GGCCTGGTGCCGCGCGGCAGCTCACACATTGAAGGCTATGAA
Forward mutagenesis primer	Primer 1, CTATTGCGAGAGAGCTGTATCAGCTGGCGTTTGACCTGCTAATCAAG	Primer 1, CCTATTGCGAGAGAGCTGTATCAGCTGACTTTTGACCTGCTAATC	CTATTGCGAGAGAGCTGTATCAGCTGGCGTTTGACCTGCTAATCAAG	TGCGAGAGAGCTGCATCAGCTGGCGTTTGACCTGCTAATCAAGTC	Primer 1, CTATTGCGAGAGAGCTGTATCAGTTCACTTTTGAC
	Primer 2, CTCCTTTGCAGCCTTGCATTCTAGCCTCAATGAAC	Primer 2, CTCCTTTGCAGCCTTGCATTCTAGCCTCAATGAAC			Primer 2, CTCCTTTGCAGCCTTGCATTCTAGCCTCAATGAAC
Reverse cloning primer	GTGGTGGTGGTGGTGCTCGAGTCAGGTGTGGAAATAGATGGGCTT
Reverse mutagenesis primer	Primer 1, CTTGATTAGCAGGTCAAACGCCAGCTGATACAGCTCTCTCGCAATAG	Primer 1, GATTAGCAGGTCAAAAGTCAGCTGATACAGCTCTCTCGCAATAGG	CTTGATTAGCAGGTCAAACGCCAGCTGATACAGCTCTCTCGCAATAG	GACTTGATTAGCAGGTCAAACGCCAGCTGATGCAGCTCTCTCGCA	Primer 1, GTCAAAAGTGAACTGATACAGCTCTCTCGCAATAG
	Primer 2, GTTCATTGAGGCTAGAATGCAAGGCTGCAAAGGAG	Primer 2, GTTCATTGAGGCTAGAATGCAAGGCTGCAAAGGAG			Primer 2, GTTCATTGAGGCTAGAATGCAAGGCTGCAAAGGAG
Cloning vector	pDNR-Lib
Expression vector	pET-28a-SUMO
Expression host	E. coli BL21 (DE3) Rosetta
Complete amino-acid sequence of the construct produced	SHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALHSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELYQLAFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ	SHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALHSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELYQLTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ	SHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELYQLAFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ	SHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQLAFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ	SHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALHSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELYQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ