Table 2. Crystallization.

Method†	Vapor diffusion
Plate type	Sitting drop
Temperature (K)	293
Protein concentration (mg ml−1)	4
Buffer composition of protein solution	50 mM HEPES pH 7.5, 150 mM Li2SO4, 10 mM DTT, 10%(v/v) glycerol, 0.1%(w/v) BOG, 0.02 mM DHT
Composition of reservoir solution	0.1 M HEPES pH 7.5, 500–800 mM sodium citrate, 20%(v/v) ethylene glycol
Volume and ratio of drop	0.75 µl protein solution:0.75 µl reservoir solution
Volume of reservoir (µl)	100

^†The crystallization method was the same for all five structures reported here.