Table 3. Data collection and processing.

Structure	L702H/H875Y/F877L/T878A	L702H/H875Y/F877L	H875Y/F877L/T878A	F877L/T878A	L702H/H875Y
PDB code	8fgy	8fgz	8fh0	8fh1	8fh2
Diffraction source	24-ID-C, APS	24-ID-E, APS	24-ID-C, APS	24-ID-C, APS	24-ID-C, APS
Wavelength (Å)	0.97895	0.97918	0.97918	0.97918	0.97918
Temperature (K)	100	100	100	100	100
Detector	Dectris EIGER2 16M	Dectris EIGER2 16M	Dectris EIGER2 16M	Dectris EIGER2 16M	Dectris EIGER2 16M
Crystal-to-detector distance (mm)	230	180	200	230	200
Rotation range per image (°)	0.2	0.2	0.2	0.2	0.2
Total rotation range (°)	140	200	200	200	250
Exposure time per image (s)	0.2	0.2	0.2	0.2	0.2
Space group	P212121	P212121	P212121	P212121	P212121
a, b, c (Å)	56.41, 66.10, 73.38	56.74, 66.08, 73.07	56.52, 65.98, 73.14	56.35, 66.06, 73.17	56.88, 65.86, 72.65
α, β, γ (°)	90, 90, 90	90, 90, 90	90, 90, 90	90, 90, 90	90, 90, 90
Mosaicity (°)	0.25	0.14	0.11	0.14	0.11
Resolution range (Å)	50–2.19 (2.33–2.19)	50–1.61 (1.70–1.61)	50–1.59 (1.69–1.59)	50–1.69 (1.80–1.69)	50–1.59 (1.69–1.59)
Total No. of reflections	75325	266841	271984	224108	338075
No. of unique reflections	14426	36469	37224	30925	37114
Completeness (%)	98.8 (99.2)	99.7 (98.7)	99.8 (99.2)	99.5 (98.1)	99.9 (99.5)
Multiplicity	5.2 (5.5)	7.3 (6.9)	7.3 (7.4)	7.2 (7.6)	9.1 (9.2)
〈I/σ(I)〉	8.8 (2.6)	25.4 (1.9)†	22.8 (2.6)	20.9 (2.0)	19.5 (2.6)
R meas (%)	19.8 (88.5)	3.5 (103)	4.0 (71.6)	4.4 (105)	6.2 (104)
CC1/2	0.992 (0.786)	1.00 (0.785)	0.999 (0.909)	0.999 (0.865)	0.999 (0.872)
Overall B factor from Wilson plot (Å2)	26	29	28	31	27

^†Note: I/σ(I) = 3.7 at 1.70 Å resolution.