Table 2. Crystallization.

Method	Hanging-drop vapor diffusion
Plate type	VDX, Hampton Research
Temperature (K)	295
Protein concentration (mg mL−1)	20
Buffer composition of protein solution	20 mM sodium HEPES pH 8.0
Composition of reservoir solution	12%(w/v) PEG 10 000, 40 mM calcium acetate pH 8.0
Volume and ratio of drop	3.8 µL protein solution plus 5 µL reservoir solution (3:4)
Volume of reservoir (µL)	400
Cryoprotectant	18% glycerol + 82% reservoir solution