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. 2023 Apr 3;61(1):621–629. doi: 10.1080/13880209.2023.2189908

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 3. — Sch B promoted the senescence of activated HSCs through induction of iron overload. (a) Liver sections stained with Perls reagents for iron deposition examination. Scale bars, 50 μm (n = 3). (b) Iron colorimetric assay of iron content in LX2 cells (n = 4). Data were expressed as mean ± SD. Significance: **P < 0.01, ***P < 0.001 vs. Control. (c) Prussian blue staining analysis of iron content in LX2 cells. Scale bar, 50 μm (n = 3). (d) SA-β-gal analysis of the senescence of LX2 cells. Data were presented as a percentage of SA-β-gal-positive cells per unit area. Scale bar, 50 μm (n = 3). (e) Determination of ROS content in LX2 cells by DCFH-DA labeling. Scale bar, 50 μm (n = 3).